July 9, 2002
Denise Paulson from GlaxoSmithKline and colleagues reported on their effort to develop a rapid and less complicated tool for assessing the status of mitochondrial DNA (mtDNA) in patients taking NRTIs for HIV infection.
Use of the real-time PCR assay with fluorogenic reporters in fat cells found a 50 percent reduction in mtDNA in fat cells from patients experiencing lipodystrophy compared to patients without lipodystrophy. When peripheral blood mononuclear cells (PBMCs) were assessed, only modest differences were noted between PBMCs from patients with lipodystrophy (n = 16; 136 genomes/cell), HIV negative controls (n = 51; 156 genomes/cell), HIV-positive patients on NRTI therapy more than six months without lipodystrophy (n = 10; 173 genomes/cell), or HIV-positive patients who were treatment naive (n = 10; 188 genomes/cell). It is unclear whether 10 percent less mtDNA in the PBMCs from patients with lipoatrophy compared to patients without lipoatrophy is significant (no statistical analysis was performed). Another poster (TuPeB4500) using a PCR-based technique also found no difference between mtDNA levels from patients on NRTI-based therapy and matched normal controls. One observer at the poster remarked that platelets can contaminate the PBMC fraction and can contribute enough mitochondria to throw the results off. More work is needed to find a blood-based assay to detect early mitochondrial toxicity. Other observers commented that PMNs may be a worthwhile fraction to study as well.
|Please note: Knowledge about HIV changes rapidly. Note the date of this summary's publication, and before treating patients or employing any therapies described in these materials, verify all information independently. If you are a patient, please consult a doctor or other medical professional before acting on any of the information presented in this summary. For a complete listing of our most recent conference coverage, click here.|
The content on this page is free of advertiser influence and was produced by our editorial team. See our content and advertising policies.